Metabolomic profiling of oesophago-gastric cancer: a systematic review
This review aims to identify metabolomic biomarkers of oesophago-gastric (OG) cancer in human biological samples, and to discuss the dominant metabolic pathways associated with the observed changes.
Selected ion flow tube-MS analysis of headspace vapor from gastric content for the diagnosis of gastro-esophageal cancer
Gastric content is a complex biofluid within the human stomach which has an important role in digestive processes. It is believed that gastric content may be a contributory factor in the development of upper gastro-intestinal diseases. In this work, selected ion flow tube mass spectrometry (SIFT-MS) has been applied to the quantification of volatile organic […]
Influence of respiratory variables on the on-line detection of exhaled trace gases by PTR-MS
Modern gas analysis techniques permit real time and on-line quantification of multiple volatile trace gases within a single exhalation. However, the influence of various respiratory manoeuvres affecting exhalation flow and the kinetics of metabolite release to the gas-phase remain largely unknown.
On-line, real time monitoring of exhaled trace gases by SIFT-MS in the perioperative setting: a feasibility study
A study is described of the first on line, real time analyses of the exhaled breath of five anaesthetized patients during the complete perioperative periods of laparoscopic surgery. These breath analyses were achieved using a selected ion flow tube, SIFT-MS, instrument, located in the operating theatre at an acceptable distance from the operating table, and coupled […]
Metabolic profiling detects field effects in nondysplastic tissue from esophageal cancer patients
The variable rate of missed cancer in endoscopic biopsies and lack of other biomarkers reduce the effectiveness of surveillance programs in esophageal cancer. Based on the “field cancerization” hypothesis that tumors arise within a transformed field with an altered biochemical phenotype, we sought to test if metabolic profiling could differentiate between histologically normal tissue from […]
Repeatability of the measurement of exhaled volatile metabolites using selected ion flow tube mass spectrometry
Selected ion flow tube mass spectrometry, SIFT-MS, has been used to determine the repeatability of the analysis of volatile metabolites within the breath of healthy volunteers, with emphasis on the influence of sampling methodology. Baseline instrument specific coefficients of variability for examined metabolites were as follows: acetone (1%), ammonia (1%), isoprene (2%), propanol (6%), ethanol […]
Variation in the levels of volatile trace gases within three hospital environments: implications for clinical breath testing
Selected ion flow tube mass spectrometry, SIFT-MS, has been used to determine the levels of volatile trace gases in the room air of three clinical environments within a busy teaching hospital. The main aims of this study were to establish background levels of trace gases and to compare them to levels typically found within exhaled […]
Selected ion flow tube mass spectrometry for targeted analysis of volatile organic compounds in human breath
The analysis of volatile organic compounds (VOCs) within breath for noninvasive disease detection and monitoring is an emergent research field that has the potential to reshape current clinical practice. However, adoption of breath testing has been limited by a lack of standardization. This protocol provides a comprehensive workflow for online and offline breath analysis using […]
A Complete Pipeline for Untargeted Urinary Volatolomic Profiling with Sorptive Extraction and Dual Polar and Nonpolar Column Methodologies Coupled with Gas Chromatography Time-of-Flight Mass Spectrometry
Volatolomics offers an opportunity for noninvasive detection and monitoring of human disease. While gas chromatography-mass spectrometry (GC-MS) remains the technique of choice for analyzing volatile organic compounds (VOCs), barriers to wider adoption in clinical practice still exist, including: sample preparation and introduction techniques, VOC extraction, throughput, volatolome coverage, biological interpretation, and quality control (QC).
Diagnostic Performance of a Noninvasive Breath Test for Colorectal Cancer: COBRA1 Study
Colorectal cancer (CRC) is the third most common cancer globally.1 When diagnosed early, the 5-year survival rate is 92%,2 yet 23% of CRCs are diagnosed at an advanced stage3 in the United Kingdom with a 5-year survival rate of 10%.2 Early CRC has symptoms that are shared with common benign conditions.4 Colonoscopy capacity is limited, and referring all symptomatic […]
